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BATF3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127 a.a) and having a molecular mass of 16.9kDa. BATF3 is fused to a 23 amino acid His-tag at
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Image Search Results
Journal: PLoS ONE
Article Title: Possible Involvement of Hepatitis B Virus Infection of Hepatocytes in the Attenuation of Apoptosis in Hepatic Stellate Cells
doi: 10.1371/journal.pone.0146314
Figure Lengend Snippet: (A) Apoptotic cell deaths were lower in LX-2 cells transfected with siRNAs against c-Jun (si-c-Jun1 and si-c-Jun2) compared with LX-2 cells transfected with siRNA-control (si-C) after incubation with conditioned media from HepG2 in the presence of MG132 (lower panel). Western blot analysis of c-Jun and GAPDH expression in LX-2 cells treated with conditioned media from HepG2 after 24 hours of MG132 treatment (upper panel). (B) Overexpression of c-Jun by the transfection of pMEKK into LX-2 cells enhanced apoptosis in LX-2 cells treated with conditioned media from HepG2.2.15 in the presence of MG132 (lower panel). Western blot analyses of c-Jun and GAPDH expression in LX-2 cells treated with conditioned media from HepG2.2.15 after 24 hours of MG132 treatment (upper panel). Apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as mean ± standard deviations of triplicate determinations. (C) Conditioned media from HepG2.2.15 (2.2.15-CM) protects hepatic stellate cells from MG132-induced apoptosis and DNA damage. Confocal microscopic findings with a high-power view (x200) of the expression of phosphorylated histone H2AX (γ-H2AX) (red), a DNA damage marker, and Annexin V (green), an apoptosis marker, in LX-2 cells treated with conditioned media from HepG2.2.15 (upper panel) or HepG2 (lower panel) in the presence of MG132.
Article Snippet: The
Techniques: Transfection, Control, Incubation, Western Blot, Expressing, Over Expression, Apoptosis Assay, Marker